Microfluidic technology has an important role in the medical field

Microfluidic chip technology platform, tumor microenvironment for the object, in the building of a closer to the physiological condition of in vitro tumor microenvironment, the research focus on the main biochemical factors of the tumor microenvironment, especially between the fibroblasts and mesenchymal stem cells, extracellular matrix and many kinds of growth factors in the important role during the process of tumor metastasis and corresponding biological mechanisms, points above are as follows: set up a kind of heterogeneous cells based on microfluidic chip new method of indirect contact with trained, to research into the interaction between fiber cells and tumor cells.



the chip micro chamber contains multiple cell culture and cell migration area, can be achieved without physical damage of cell culture and cell movement of real-time monitoring, and to integrate the unit operation of cells into a single chip. The results showed that the fibroblasts in the role of tumor cells induced, directional migration, at the same time, fibroblasts are activated and transdifferentiation. Development of a series can be used for 2 d and 3 d cell culture functional bionic microfluidic chip, is used to study between mesenchymal stem cells ( MSCs) In salivary gland adenoid cystic cancer cells ( ACC - M) The role of micro environment, and MSCs and ACC - is discussed in this paper M interaction occurs may be involved in signal transduction pathways. Based on this method, accurately realize the regional distribution of cells and training; A controlled form concentration gradient of soluble factors; At the same time, cell migration analysis can be quantified and real-time. The results show that 1) The ACC - MSCs The entrainment of M cells directional migration, this process may be by the ACC TGF - M cells β Mediated by the. 2) MSCs can destroy the ACC - Connection between M cells, significantly reduce its E - Cadherin expression, and in the role of CXCL12 concentration gradient, promote the ACC - M cell invasion and metastasis, and further reveals the CXCL12 / CXCR4 signaling pathways in MSCs promote ACC - M important participation in the process of transfer.



set up a new type of microfluidic chip based cell migration dynamic monitoring method, to study tumor cell invasion to the dynamic process of three-dimensional extracellular matrix and the related biological behavior changes in the process of spontaneous strike. The method can real-time monitoring of cell movement, at the same time can be achieved to the initial position of cell movement accurate positioning, quantitative biology can get more information. The results showed that the tumor cells HepG - 2 in the process of invasion of extracellular matrix, through the interaction with the extracellular matrix, cytoskeleton reorganization and cell morphology change, promote the process of tumor invasion.


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