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Microfluidic weapon - laser equipment production experiment Microfluidic chip cartilage
by：Transon
2021-01-08
The production of microfluidic test chip for laser equipment factory laser equipment, the company equipment has cooperation with universities laboratory, was published in microfluidic academic BBS test papers, make the chip of the vast majority of customer recognition.
due to an ageing population, osteoarthritis ( osteoarthriti) The common diseases caused by the social impact is expected to be a sharp increase in its common treatments for pain relief or surgical treatment. OA treatment, due to the lack of accurate model of preclinical O in traditional cultivation and 3 2, both cannot be accurately simulated dynamic culture microenvironment of cartilage cells, as well as the joint activities, cartilage cells by specific stimuli, such as continuous nutrition and specific mechanical stimulation. And the introduction of microfluidic technology, through the microfluidic control and with & quot; Layered & quot; Microfluidic chip structure of cartilage, for this a series of problems brought new innovative solutions. In this paper, a microfluidic chip and cartilage cell culture microfluidic system, the paper use microfluidic means for the cartilage cell culture experiment mechanism of microfluidic chip cartilage
covers joint articular cartilage bone rubbing surface, smooth and elastic, its main function is to promote the load transfer between the bones, for joint activities provide a buffer and protection. Articular cartilage is composed of mesenchymal cells and chondrocytes, structure without blood vessels and nerves, nutrients by the stromal cells to chondrocytes, joint activities, the mechanical stimulation will promote the transfer of nutrients.
a can to a certain extent, to simulate the above condition of core of microfluidic cartilage.
the graph is chip vertical view, top drive cavity, and the positive pressure FlowEZ pressure or negative pressure Flow EZ pressure pump connected; Middle yellow part for cell culture chamber, for cartilage cell culture, training for hydrogel matrix; Below the red part for irrigation circulation way, simulation of blood vessels, cartilage cells nutrients. Combined with the chip cross section diagram b, can be found that the cell culture chamber side is with elastic PDMS membrane, PDMS elastic membrane and three drivers are linked together, for a lot of micro column array, the other side connected to the filling and circulation way. Chips work, through the three ShiJiaZheng driven cavity pressure or negative pressure, thereby to different mechanical stimulation of PDMS elastic membrane, PDMS elastic membrane has different degree of deformation, when that simulation in articular cartilage cells activity of extrusion and & quot; Rebound & quot; 。
cartilage simulation microfluidic system
system USES positive pressure FlowEZ pressure or negative pressure Flow EZ pressure pump as the driving source, with the chip of 3 ShiJiaZheng driven cavity pressure and negative pressure, and 2 to 3 switch valve is used to realize the pressure switch, the operation process is controlled by PC software automation programming. On a chip, using microscope observation and analysis of the deformation of chip PDMS elastic membrane, and cartilage cell culture form. Below part the result of the experiment is taken part of PDMS elastic membrane microscope images, respectively, shows the PDMS elastic membrane in 4 different pressure circumstances occur deformation.
ShiJiaZheng pressure, when pressure is 800 mbar, exert negative pressure, the pressure to - 350mbar.
in the past few years, the transition of the traditional 2 d cultivation to the 3 d, is a big progress in vitro culture system. 3 d training can induce phenotype, proliferation and differentiation in gene expression rate, a higher physiological relevance, but compared with the living organ in the body, most cells by chemical and mechanical stimuli, 3 d culture model is still seem to be very simple. And microfluidic technology, it is the powerful tools for the complexity of living organism. Examples in this article has a certain universality, can be applied to the intestinal chip, chip of the esophagus and similar research.
due to an ageing population, osteoarthritis ( osteoarthriti) The common diseases caused by the social impact is expected to be a sharp increase in its common treatments for pain relief or surgical treatment. OA treatment, due to the lack of accurate model of preclinical O in traditional cultivation and 3 2, both cannot be accurately simulated dynamic culture microenvironment of cartilage cells, as well as the joint activities, cartilage cells by specific stimuli, such as continuous nutrition and specific mechanical stimulation. And the introduction of microfluidic technology, through the microfluidic control and with & quot; Layered & quot; Microfluidic chip structure of cartilage, for this a series of problems brought new innovative solutions. In this paper, a microfluidic chip and cartilage cell culture microfluidic system, the paper use microfluidic means for the cartilage cell culture experiment mechanism of microfluidic chip cartilage
covers joint articular cartilage bone rubbing surface, smooth and elastic, its main function is to promote the load transfer between the bones, for joint activities provide a buffer and protection. Articular cartilage is composed of mesenchymal cells and chondrocytes, structure without blood vessels and nerves, nutrients by the stromal cells to chondrocytes, joint activities, the mechanical stimulation will promote the transfer of nutrients.
a can to a certain extent, to simulate the above condition of core of microfluidic cartilage.
the graph is chip vertical view, top drive cavity, and the positive pressure FlowEZ pressure or negative pressure Flow EZ pressure pump connected; Middle yellow part for cell culture chamber, for cartilage cell culture, training for hydrogel matrix; Below the red part for irrigation circulation way, simulation of blood vessels, cartilage cells nutrients. Combined with the chip cross section diagram b, can be found that the cell culture chamber side is with elastic PDMS membrane, PDMS elastic membrane and three drivers are linked together, for a lot of micro column array, the other side connected to the filling and circulation way. Chips work, through the three ShiJiaZheng driven cavity pressure or negative pressure, thereby to different mechanical stimulation of PDMS elastic membrane, PDMS elastic membrane has different degree of deformation, when that simulation in articular cartilage cells activity of extrusion and & quot; Rebound & quot; 。
cartilage simulation microfluidic system
system USES positive pressure FlowEZ pressure or negative pressure Flow EZ pressure pump as the driving source, with the chip of 3 ShiJiaZheng driven cavity pressure and negative pressure, and 2 to 3 switch valve is used to realize the pressure switch, the operation process is controlled by PC software automation programming. On a chip, using microscope observation and analysis of the deformation of chip PDMS elastic membrane, and cartilage cell culture form. Below part the result of the experiment is taken part of PDMS elastic membrane microscope images, respectively, shows the PDMS elastic membrane in 4 different pressure circumstances occur deformation.
ShiJiaZheng pressure, when pressure is 800 mbar, exert negative pressure, the pressure to - 350mbar.
in the past few years, the transition of the traditional 2 d cultivation to the 3 d, is a big progress in vitro culture system. 3 d training can induce phenotype, proliferation and differentiation in gene expression rate, a higher physiological relevance, but compared with the living organ in the body, most cells by chemical and mechanical stimuli, 3 d culture model is still seem to be very simple. And microfluidic technology, it is the powerful tools for the complexity of living organism. Examples in this article has a certain universality, can be applied to the intestinal chip, chip of the esophagus and similar research.
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