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Microfluidic chip optical methods detect myocardial markers
by：Transon
2021-01-08
Using high precision laser engraving machining microfluidic chip can complete the test!
at present for the detection of myocardial markers, mostly based on antigen antibody immune response. Biologists often used fluorescent material antigens or antibodies or enzymes standard, and then from the reaction products of fluorescence intensity, the substrate colorimetric and capture chemiluminescence substrate light to quantify the concentration of the detected objects. Substrate visible colorimetric method for the sensitivity is relatively low, generally can meet the test requirements, low concentration of myocardial markers application is less. The fluorescence immunoassay method with high sensitivity and adopted by most researchers.
for microfluidic chip micro level detection channel, fluorescence immunoassay methods sometimes can not satisfy the requirement of the low concentration of myocardial marker detection. Therefore, it is creative design of the concentration of fluorescent markers team reaction pool and embedded photomultiplier tube detection device, implementation of c-reactive protein ( C - 反应蛋白(CRP) The low concentration detection. The specific experimental process is as follows: first of all produced with 1 mm & times; 0. 8毫米× 30 um preconcentration reaction pool T polydimethylsiloxane ( Polydimethylsiloxane,PDMS) Chip, enrichment in physical reaction pool design microstructure CRP antibodies by magnetic beads; Then fluorescence labeled antigen of CRP and CRP antigen injection reaction pool, to be detected after incubation elution fluorescent tags; Finally in the reaction pool downstream microchannel using embedded photomultiplier tube fluorescent signal detection, the results measured CRP the minimum detection limit of 1. 4 nmol/L. This method can effectively improve the detection sensitivity, but the drawback is that magnetic beads modified antibody and antigen of fluorescent tags operation must be outside the chip, testing time is relatively long.
at present for the detection of myocardial markers, mostly based on antigen antibody immune response. Biologists often used fluorescent material antigens or antibodies or enzymes standard, and then from the reaction products of fluorescence intensity, the substrate colorimetric and capture chemiluminescence substrate light to quantify the concentration of the detected objects. Substrate visible colorimetric method for the sensitivity is relatively low, generally can meet the test requirements, low concentration of myocardial markers application is less. The fluorescence immunoassay method with high sensitivity and adopted by most researchers.
for microfluidic chip micro level detection channel, fluorescence immunoassay methods sometimes can not satisfy the requirement of the low concentration of myocardial marker detection. Therefore, it is creative design of the concentration of fluorescent markers team reaction pool and embedded photomultiplier tube detection device, implementation of c-reactive protein ( C - 反应蛋白(CRP) The low concentration detection. The specific experimental process is as follows: first of all produced with 1 mm & times; 0. 8毫米× 30 um preconcentration reaction pool T polydimethylsiloxane ( Polydimethylsiloxane,PDMS) Chip, enrichment in physical reaction pool design microstructure CRP antibodies by magnetic beads; Then fluorescence labeled antigen of CRP and CRP antigen injection reaction pool, to be detected after incubation elution fluorescent tags; Finally in the reaction pool downstream microchannel using embedded photomultiplier tube fluorescent signal detection, the results measured CRP the minimum detection limit of 1. 4 nmol/L. This method can effectively improve the detection sensitivity, but the drawback is that magnetic beads modified antibody and antigen of fluorescent tags operation must be outside the chip, testing time is relatively long.
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